By Herbert Begemann
-Enhanced sixth variation includes extra illustrations and documented figures
-A excellent reference for hematological and oncological departments and the clincal labortory
-Classified findings from the area future health Organization
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Additional resources for Atlas of Clinical Hematology
Wigzell. : Lymphopenia and change in distribution of human Band T lymphocytes in peripheral blood induced by irradiation for mammary carcinoma. Lancet 1, 1352 (1972) solid tumors, especially in advanced stages. The number of T lymphocytes also decreases with progressing age. Demonstration of Hemoglobin F in Erythrocytes (Kleihauer and Betke) Acridine Orange Stain for Fluorescene Microscopy Principle. 3 at 37° C. HbF remains in the erythrocytes and can be visualized by staining. Fluorescence microscopy is frequently used for the cytodiagnosis of tumor cells after staining with acridine orange.
Staining the "Thick Drop" Let one or two drops of blood fall onto a slide and spread with a small lancet to the size of a dime (not too thick, otherwise the "thick drop" will peel off during drying or float away during staining). Dry in air and stain only when completely dry. Staining is performed without fixation. The "thick drop" method is a concentration method to detect even minor infestation with parasites. Staining without fixing produces hemolysis. The erythrocytes are destroyed and the parasites are thus dislodged from them and demonstrated.
Also typical of eosinophil leukocytes is that the segmented forms mostly have only two, less often three nuclear segments. Mitoses of eosinophil granulocytes are occasionally observed in the bone marrow. All eosinophils are peroxidase-positive. The basophils also follow the same development (cell 10-14) but they can usually be differentiated only from the myelocyte stage on. Typical is the coarse basophil granulation that more or less conceals the cytoplasm, including the nucleus. The nucleus of segmented basophils is polymorphous and often cloverleaf shaped.
Atlas of Clinical Hematology by Herbert Begemann